Experimental study on related factors 1 gene of X-chromosome linked inhibitor of apoptosis on inhibiting cell proliferation and inducing apoptosis in hepatocellular cell line
- VernacularTitle:X-染色体相关凋亡抑制蛋白相关因子1抑制肝癌细胞增殖和诱导凋亡的实验研究
- Author:
Dong-Mei SHI
;
Tian-Le MA
;
Shui-Ping TU
;
- Publication Type:Journal Article
- Keywords:
Related factor of X-chromosome linked inhibitor of apoptosis protein;
Hepatocellular carcinoma;
Apoptosis
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and the effects of X-chromosome linked inhibi- tor of apoptosis (XIAP) associated factor 1 (XAF1) on apoptosis and cell proliferation in SMMC7721 hepatocellur carcinoma (HCC) cell line.Methods The expression of XAF1 mRNA and protein in hu- man SMMC7721 cell line were detected by semi-quantitative,RT-PCR and Western blot.Plasmid con- structs expressing sense and antisense XAF1 were generated and transfected into SMMC7721 cell line to establish stable transfectants.Cell proliferation and apoptosis were detected by MTT,flow cytometry and TUNEL.Results XAF1 mRNA and protein were detectable in SMMC7721 cell line but lower than that in normal liver cell.Stable expression of XAF1 significantly inhibited cell proliferation and increased spontaneous apoptosis in SMMC7721 cell (P＜0.05).Over-expression of XAF1 in stable transfactants increased the sensitivity of SMMC7721 cell to chemotherapeutic drugs such as 5-fluorouracial and hydroxycamptothecin.Conclusions Over-expression of XAF1 induces apoptosis and inhibits SMMC7721 cell growth.XAF1 may be a promising candidate for HCC gene therapy.
