Activation of JAK/STAT1 signal transduction pathway in different organs of MRL/lpr lupus mice
- VernacularTitle:JAK/STAT1信号转导途径在MRL/lpr狼疮鼠不同器官中的活化研究
- Author:
Jing DONG
;
Qi-Xin WANG
;
Shao-Chun WANG
;
Xian-Fu MA
;
Xiu-Ling JIA
;
Qing-Rui YANG
;
Yuan-Chao ZHANG
;
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus;
systemic;
Mice;
inbred MRL lpr;
Lupus nephritis;
Signal transducer and activator of transcription
- From:
Chinese Journal of Rheumatology
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the activation and function of Janus protein-tyrosine kinase (JAK)/ signal transducer and activator of transcription (STAT1) signal transduction pathway in kidney,lung and brain of MRL/lpr mice.Methods MRL/lpr mice with systemic lupus erythematosus (SLE) were studied at the age of 12 weeks up.Non-SLE MRL/lpr mice were used as controls.We used phosphospecific antibodies to detect STAT1 activation in kidney,lung and brain by immunohistochemistry and Western blots.Gene expression of the STAT induced feedback inhibitors of cytokine signaling 1 (SOCS-1) was investigated by SYBR green I real-time reverse transcriptase polymerase chain reaction (PCR).Results Phosphorylation of STAT1 protein was markedly activated in these three organs,although renal and pulmonary STAT1 activation were much more evidently activated.SOCS-1 gene expression increased in all three organs,while renal SOCS-1 gene expres- sion increased less than lung and brain.Conclusion The activation of JAK/STATI signal transduction path- way may be pathogenic in the organ involvement and progression of SLE.The pathogenesis of lupus nephritis may also be associated with the down-regulation of SOCS-1 feedback inhibition.
