Effects of estrogen receptor ? on proliferation of ovarian clear cell adenocarcinoma ES-2 in vitro and in vivo
- VernacularTitle:雌激素受体?在卵巢透明细胞癌细胞增殖中的作用
- Author:
Jing ZHU
;
You-Ji FENG
;
- Publication Type:Journal Article
- Keywords:
Receptors,estrogen;
Ovarian neoplasms;
Adenoearcinoma,clear cell;
Cell division;
Transfection
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the involvement of estrogen receptor(ER)? in the proliferation of ovarian clear cell adenocarcinoma by restoring ER? expression in a cell line ES-2.Methods A plasmid with full length ER? cDNA,pRSV-ER? and its negative vector control pRSV were introduced into ES-2.The cells transfected were named according to the plasmids:ES-pRSV,ES-pRSV-ER?.RT-PCR and western blot were used to detect the expression of ER? in ES-2,ES-pRSV and ES-pRSV-ER? cells.The growth activities of cells in vitro were detected by methyl thiazolyl tetrazolium(MTT)assay,and in vivo growth in nude mice was also observed.Flow cytometry was performed to show the change of cell cycles. Results The ES-pRSV-ER? cells were identified with ER? mRNA and protein expression.The growth activities of ES-pRSV-ER? were inhibited in vitro.In MTT analysis,the values of ES-2,ES-pRSV,and ES- pRSV-ER? cells were 0.78?0.05,0.81?0.06,and 0.53?0.07(the third was lower compared with the former two,P
