Coordinative inhibitory effect of p15~(INK4B) gene transfection combined with arsenic trioxide on human squamous esophageal carcinoma
- VernacularTitle:p15~(INK4B)基因转染联合亚砷酸对人食管鳞癌的协同抑制作用
- Author:
Tie-Fu LIU
;
Xue-Yan ZHANG
;
Li-Ping SUN
;
Yang YU
;
- Publication Type:Journal Article
- Keywords:
p15~(1NK4B)gene;
Gene transfection;
Arsenic trioxide;
Esophageal carcinoma
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of combined treatment of p15 gene transfection with arsenic trioxide(As_2O_3)on proliferation and apoptosis of human squamous esophageal carcinoma cell line EC109.Methods Plasmid pcDNA3.1(+)-p15 was introdueted into EC109 cell hy the lipo- somes.As_2O_3(2?mol/L)was added to stable transfeeted cells for succedent experiments.The existence of exogenous p15 gene eDNA and the expression of P15 protein were assayed by PCR and Western blotting,respectively.The proliferation and apoptosis were measured by means of MTT,colony forma- tion assay,transmission electron microscopy,The cell cycle and population of apoptosis were measured by flow cytometry.Results After transfection,p15 gene mRNA and protein expressed in EC109 cells. Combined p15 transfection with As_O_3,the EC109 cell growth and colony formation were significantly decreased(P＜0.05,P＜0.01),compared with either p15 transfected group or treated with As_2O_3 group.After combining p15 transfection with As_2O_3 for 3 days,EC109 cell cycle was more arrested at G_1/S.The population of G_1 phase cells was significantly increased(P＜0.05),the population of S phase cells was significantly decreased(P＜0.05),and the population of the apoptosis cells was significantly increased(P＜0.01),compared with either p15 transfected group or treated with As_2O_3 group.More obvious apoptosis was found in the group with combined treatment of p15 gene transfection and As_2O_3 by transmission electron microscope.Conclusion p15 gene transfection combined with As_2O_3 show a signifi- cant effect on enhancing proliferation inhibition and could induce more apoptosis on EC109 cells in vitro.
