The expression of interferon-regulatory factor genes in patients with systemic lupus erythematosus
- VernacularTitle:系统性红斑狼疮患者干扰素调节因子IRF1 IRF4 IRF8基因表达的研究
- Author:
Jie QIAN
;
Nan SHEN
;
Gui-Mei GUO
;
Nian-Hong WAN
;
Yan LIN
;
Xin-Fang HUANG
;
Hui WU
;
Shun-Le CHEN
;
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Polymerase chain reaction;
Interferon-regulatory factor
- From:
Chinese Journal of Rheumatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether the expression of interferon-regulatory factor genes are re- lated to systemic lupus erythematosus (SLE).Methods The clinical data of 45 SLE patients and 37 normal controls were collected.Total RNA of peripheral blood was extracted and transcripted into cDNA.Sybr green dye based real-time quantitative PCR method was used to compare the expression (indicated as-??Ct value) of IRFI,IRF4,IRF8 in patients with SLE and those in the controls.Results The levels of IRF1,IRF4 and IRF8 mRNA were-3.90?0.19,-8.04?0.25 and 3.60?0.15 respectively in normal controls.In SLE patients, IRF4 mRNA expression was -8.82?0.18,higher than that in normal (P=0.011).But IRF8 mRNA expression was 3.09?0.13,lower than that in normal (P=0.012).Conclusion Abnormal IRF mRNA expression is found in the peripheral blood of SLE patients.IRFs may play roles in the pathogenesis of SLE by affecting the differen- tiation of Th cells.
