Granulocyte colony-stimulating factor gene polymorphism in patients with systemic lupus erythematosus in Northern Chinese
- VernacularTitle:中性粒细胞集落刺激因子基因多态性与系统性红斑狼疮相关性的研究
- Author:
Yan-Qiu LI
;
Xiao-Li LI
;
Li-Ning WANG
;
Yu WANG
;
Xiao-Dan LIU
;
Li LI
;
Zan-Mei QI
;
Yi JIANG
;
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Granulocyte colony-stimulating factor;
Polymorphism,single nucleotide
- From:
Chinese Journal of Rheumatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the polymorphism(s) or mutation(s) of the granulocyte colony-stim- ulating factor gene (G-CSF,Csf3) and its possible association with the susceptibility to systemic lupus erythe- matosus (SLE).Methods Polymorphism screening was carried out by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP),using DNA from 204 Northern Chinese patients with SLE,459 ethnically matched healthy control,78 patients with other autoimmune diseases.Results It was i- dentified that 4 polymorphisms at position 1869,1205,1931,and 394.The nucleotide sequences of the sample that showed different SSCP patterns and different distribution between SLE and healthy controls were deter- mined by direct sequencing.Only the distribution of the fifth extron of Csf3 (1931) between SLE and controls was different.The frequency of A/G genotype (60.5%) and A allele (53.2%) at position 1931 in patients with SLE was significantly higher than that in healthy controls (51.6% and 45.2%,respectively),whereas,the fre- quency of G/G genotype (16.2%) and G allele (46.8%) in patients with SLE was lower than that in healthy controls (28.9% and 54.8% respectively).Conclusion The results suggest that Csf3 (1931) is significantly associated with the susceptibility to SLE in Northern Chinese patients .Further population and functional stud- ies will be followed to establish Csf3 (1931) as one of the susceptible genes for SLE.
