Effects of dexamethasone on absorption of lung edema in rabbits with seawater drowning-induced acute lung injury
- VernacularTitle:地塞米松对海水淹溺性肺损伤兔肺水肿吸收的影响
- Author:
Xinmin DING
;
Yunyou DUAN
;
Chaosheng PENG
;
Huasong FENG
;
Zhiqing XUE
;
Jiguang MENG
;
Qinzhi XU
;
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Lung edema;
Drowning,Seawater;
Dexamethasone
- From:
Chinese Journal of Emergency Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of dexamethasone on absorption of lung edema in rabbits with seawater drowning-induced acute lung injury.Methods Seawater(4 ml/kg body weight)was instilled into the lower trachea of ventilated and anesthetized rabbits.These rabbits were assigned randomly to receive intravenous injection of 1 mg/ kg body weight of dexamethasone(dexamethasone group,DG)or 2 ml of normal saline(control group,CG).Lung edema was measured by extravascular lung water index(EVLWI)using a gravimetric method.Three hours after treatment, epithelial Na~+ channel subunit-?(?-ENaC)mRNA and Na~+/K~+-adenosine triphosphatase subunit-?l(NKA-?l) protein abundances in lung tissues were respectively measured by reverse transcriptase-polymerase chain reaction and Western blotting,and NKA activity was measured by monitoring the release of inorganic phosphate(Pi)from adenosine triphosphate(ATP).Results The DG's EVLWI was significantly lower than the CG's[(0.508?0.089)vs.(0.648?0.102),P＜0.05)],but the DG's NKA activity,?-ENaC mRNA and NKA-?l protein abundances were significantly higher than the CG's,correspondingly(P＜0.05).Conclusions With up-regulation of the NKA activity and expressions of?-ENaC and NKA-?l,dexamethasone treatment could promote the absorption of lung edema in rabbits with seawater drowing-induced acute lung injury.
