Role of antibody chin in analysis of inflammatory cytokine expression in severe sepsis
- VernacularTitle:抗体芯片分析炎性细胞因子在严重脓毒症中的表达
- Author:
Jing YAN
;
Zhaocai ZHANG
;
Guolong CAI
;
Yihua YU
;
Caibao HU
;
Liang WU
;
- Publication Type:Journal Article
- Keywords:
Antibody chip;
Sepsis;
Cytokine;
Inflammation;
Protein array
- From:
Chinese Journal of Emergency Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of inflammatory cytokines in patients with severe sepsis by using an antibody chip.Methods Twelve patients with severe sepsis and other 10 patients whose age and gender were matehed were enrolled in this study.Proteins from patients were labeled with biotin.The biotin-labeled proteins reaeted with antibody chips,on which there were antibodies of 40 major inflammatory cytokines.The target proteins were conjugated with streptomycin antibody labeled by horseradish peroxidase(HRP),and signals were imaged by laser scanner.Results In comparison with control group,the serum levels of inflammatory eytokines ineluding pro-and anti-inflammatory cytokines,ehemokines and certain eytokines receptors were notably increased,while expression of anti-inflammatory interleukin(IL)-2,-4,-13,-15 was remarkably decreased in sepsis group.Conclusions Excess inflammatory response and imbalance of pro-and anti-inflammatory eytokines were presented in the eourse of severe sepsis.
