Protective effect of Ginaton on lipopolysaccaride-induced acute lung injury in rats
- VernacularTitle:金钠多对脂多糖所致急性肺损伤大鼠的保护作用
- Author:
Guohua LU
;
Renfei DAN
;
Jianying ZHOU
;
Hangping YAO
;
- Publication Type:Journal Article
- Keywords:
Lipopoly saccharides (LPS);
Acute lung injury (ALI);
Ginaton;
Nuclearfactor-?B (NF-?B);
serum
- From:
Chinese Journal of Emergency Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ginaton on acute lung injury (ALI) induced by lipopolysaccaride (LPS).Methods The acute lung injury rat model was induced by receiving 5 mg/kg LPS injection in tail vein.A total of 63 Wistar rats were divide into 3 groups without caring sex.Saline control group,LPS treated group and Ginaton treated group.The samples of different groups were collected 2 h,6 h,and 10 h after tail vein administration.Serum soluble cell ashesion molecule-1 (sICAM-1) was measured by ELISA,immunohistochemical staining and Western blotting of NF-kB were performed on sections of lung specimens.Results The acute lung injury rat model was successfully induced by receiving LPS injection in tail vein.The sICAM-1 levels increased more in Ginaton treated group than those in Saline control group (P＜0.01),and the increase in LPS treated group were the highest.By immunohistochemical staining,the positive NF-kB cells in Ginaton treated group were much less than those in LPS treated group (P＜0.01),and in Saline control group were least (P＜0.01).The results of the Western-blot method were consistent with immunohistochemical method.Conclusion ALI could be induced by LPS,Ginaton showed a protective effect through probably inhibiting activation of NF-kB on LPS-induced-ALI in this animal model.
