Effects of S100A4 expressed In white matter astrocytes on sensory neurite outgrowth in vitro
- VernacularTitle:白质星形胶质细胞内S100A4蛋白对小鼠背根神经节细胞突起生长的影响
- Author:
Zheng-Yu FANG
;
Liang XIONG
;
Xiao-Lin HUANG
;
Kozlova ELENA
;
Jian-Zhi WANG
;
- Publication Type:Journal Article
- Keywords:
Nerve regeneration;
Dorsal root ganglion;
Calcium-binding protein;
siRNA;
Cell culture
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of white matter astrocytes and their specific protein S100A4 in sensory neurite outgrowth in vitro.Methods White matter astrocyte cultures expressing S100A4 were prepared. Dissociated adult dorsal root ganglion(DRG)cells were placed on the top of the astrocytes and co-cultured for 6,12, 18,24 hours.Small interfering S100A4 RNA was used to eliminate S100A4 expression.The growth of DRG cell neu- rites on S100A4-sileneed and S100A4-expressing astrocytes was compared.Results 12,18 and 24 hours after the co-culture with S100A4-expressing or S100A4-silenced astroeytes,neurite growth from the DRG cells was observed. Neurite outgrowth was significantly greater in S100A4 siRNA treated cultures compared to control siRNA treated white matter astrocyte cultures.Conclusion These findings suggest that white matter astroeytes are able to support axonal regeneration and,furthermore,that administration of small interfering S100A4 RNA provides strong additional support for axon regeneration.
