Association of tumor necrosis factor polymorphisms with susceptibility to ulcerative colitis in Chinese Han population
- VernacularTitle:肿瘤坏死因子基因多态性与溃疡性结肠炎相关性研究
- Author:
Qian CAO
;
Min GAO
;
Gang ZHOU
;
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Tumor necrosis factor;
Gene polymorphism
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between tumor necrosis factor(TNF) promot- er polymorphisms and susceptibility to ulcerative colitis(UC) in the Chinese Han population.Methods Blood samples from 110 unrelated UC patients and 292 healthy controls from Zhejiang Province,Eastern China were studied.Genotyping for 6 common TNF promoter polymorphisms (TNF—1031T/C,—863C/A,—857C/T,—380G/A,—308G/A,—238G/A)was carried out using polymerase chain se- quence-specific primer (PCR-SSP).Results TNF—308A was associated with susceptibility to UC (al- lele frequency patients 14.6% vs.controls 8.9%,P=0.02).TNF—857T was increased in patients but did not reach statistical significance (allele frequency 17.3% vs.12.2%,P=0.06).Haplotype analysis revealed 6 haplotypes including two (H5 and H3) which contained TNF-308A.H5 was associ- ated with disease (haplotype frequency patients—12.3% vs.controls 7.5%,P=0.03).Homozygosity for the haplotype H4 comprising the common alleles at each TNF promoter SNP was negatively associat- ed with disease (patients vs.controls 24.5% vs.34.9%,P＜0.05).Conclusions We report first the association of TNF-308A polymorphisms with UC in Chinese patients.The functional study of the poly- morphisms in Chinese Han population is required.
