Significance of positive expressin of bcl-2 and bax in osteoarthritis in rabbit chondrocyte
- VernacularTitle:兔骨关节炎软骨细胞中bcl-2、bax mRNA及蛋白表达的意义
- Author:
Si-Jian LU
;
Ling SHAN
;
Hua-Dong ZHANG
;
Fen-Fen WANG
;
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Chondrocytes;
Genes;
bcl-2;
Genes;
bax
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the significanec of genes bel-2 and bax in chondrocyte apoptosis in experimental osteoarthritis in rabbits.Method Twelve New Zealand rabbits divided into two groups at random:model group and control group.Model group with osteoarthritis was duplicated by immobilizing with gypsum at extension position in right hind limb of rabbits.Rabbits of model group were executed after 6 weeks and chondrocytes were taken.Positive expression rate of bcl-2 and bax mRNA was measured by immunohisto- chemistry and Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).Results Positive expression rate of mRNA in bel-2 and bax of model group was obviously more than control group(P＜0.05).According to immunohistochemistry,and grey grade of positive expression of protein in bcl-2 and bax of chondrocytes in model group was lower and positive expression rate was gigher compared with control group (P＜0.05,P＜0.01).bcl-2 and bax of model group were lower than control group(P＜0.05).Conclusion Genes bcl-2 and bax participate and thus accelerate chondrocyte apoptosis of osteoarthritis together.
