Experimental study of acute lung injury induced by ventilator in rats
- VernacularTitle:呼吸机致大鼠急性肺损伤的实验研究
- Author:
Xinri ZHANG
;
Yongcheng DU
;
Hongying JIANG
;
Jianying XU
;
Yongjian XU
;
Guoqiang YANG
;
Jing FENG
;
Mingkai LI
;
Xiaoyan ZHANG
- Publication Type:Journal Article
- Keywords:
Mechanical ventilation;
Acute lung injury;
Myloperoxidase;
Bronchoalveolar lavage fluid
- From:
Chinese Journal of Emergency Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different tidal volume ventilation on acute lung injury in rats. Methods Thlrty-two normal Wistar rats were randomly divided into four groups:control group,low tidal volume group(L- V_T),conventional tidal volume group(C-V_T)and high tidal volume group(H-V_T).The pathologic changes of the lungs were observed under macrography,light and electron microscope.The blood gas analysis(PaO_2),the counts of neutrophils (PMN),the levels of protein and the myloperoxidase(MPO)activities in bronchoalveolar lavage fluid(BALF)were measured by biochemical methods respectively.Results There were no distinct pathological differences between L-V_T group and control group under macrography,light and electron microscope.In the C-V_T and H-V_T groups,there were different degree of lung injuries under light and electron microscope,their PMN,MPO activity and protein level in BALF were significantly higher than those of control and L-V_T groups and their PaO_2 were significantly lower than those of control and L- V_T groups(P＜0.01,P＜0.05).The MPO activity and the protein level in BALF were also significantly higher than those of C-VT group(P＜0.01)Of the above indexes,there were no statistical differences between L-V_T group and control group(P＞0.05).Conclusion Conventional tidal volume ventilation alone,without any lung-protective strategy, could produce injuries to the normal lung tissues,while low tidal volume ventilation hadn't effects on them.The injury effects produced by mechanical ventilation was closely related to the recruitment and activation of neutrephils in the lung.
