The effect of hyperbaric oxygen therapy on apoptosis:Expression of Bcl-2 and Bax protein in rats with traumatic brain injury
- VernacularTitle:高压氧对脑外伤大鼠海马神经元凋亡的影响
- Author:
Jing CHEN
;
Chun-Jing YOU
;
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen therapy;
Traumatic brain injury;
Bcl-2;
Bax;
Rats
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of hyperbaric oxygen therapy on traumatized cerebral neurons and investigate its mechanism in attenuating cerebral damage.Methods Seventy-two rats were randomly assigned to a control group,a traumatic brain injury group or a traumatic brain injury group treated with hyperbaric oxygen. Each group was observed 1,7 and 24 days after the operation.TUNEL was used to examine the distribution of apop- tosis cells.An immunohistoehemical method was used to examine the distribution of Bcl-2 and Bax immunoreactive- positive cells in the brain tissues.Results The average percentages of both apoptosis cells and Bcl-2 immunoreac- tive-positive cells were lower in the CAl region of the traumatized brains treated with hyperbaric oxygen than in those of the traumatic injury group at each time of observation.A significant difference in Bax immunoreactive-positive cells between the two groups was also observed.Conclusion Hyperbaric oxygen therapy can significantly protect neurons against traumatic brain injury and modulate the expression of the apoptosis related genes Bcl-2 and Bax.This may ex- plain the protective mechanisms of hyperbaric oxygen therapy in treating traumatic brain injury.
