Study on mechanism of inhibitory effect of carboxymethyl-chitosan on the chondrocytes apoptosis induced by interleukin-1?
- VernacularTitle:羧甲基壳聚糖抑制白细胞介素-1?诱导的软骨细胞凋亡及作用机制的研究
- Author:
Qing CHEN
;
Shi-Qing LIU
;
Yu-Min DU
;
Hao PENG
;
Yang YU
;
Li-Ping SUN
;
- Publication Type:Journal Article
- Keywords:
Chitosan;
Interleukin I;
Chondrocytes;
Apoptosis
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of carboxymethyl-chitosan(CM-chitosan)on chondro- cyte apoptosis induced by recombinant human interleukin-1?(rhIL-1?)and explore its mechanism.Methods Rabbit chondrocytes were isolated and cultured.Chondrocytes were pretreated with different concentrations of CM-chitosan for 1 h,then 10 ng/ml IL-1?were added into the culture medium.After 24 h,the apoptotic rates of chondrocytes were measured by flow cytometry with AnnexinⅤ-FITC and PI staining.The morphology of nuclei was observed by fluorescent microscopy with Hoechst 33342 staining.The mitochondrial membrane po- tentials were tested by confocal laser scanning microsocopy with Rhodamine-123 and ATP contents were mea- sured by luciferase reaction.Results CM-chitosan could inhibit chondrocyte apoptosis and restore the func- tion of mitochondria induced by IL-113 in a dose-dependent manner.Conclusion CM-ehitosan can inhibit chondrocyte apoptosis by protecting mitochondrial function.
