The expression and significance of human macrophage metalloelastase in both gastric cancer cell lines and gastric cancer tissues
- VernacularTitle:人巨噬细胞金属弹性蛋白酶在胃癌细胞株及胃癌组织中的表达及意义
- Author:
Hong ZHANG
;
You-Ming LI
;
Chun-Xiao CHEN
;
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Human macrophage metalloelastase;
Gene;
Protein
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of human macrophage metalloelastase(HME) both in gastric cancer cell lines and gastric cancer tissues,and to find the role of HME in gastric carcino genesis.Methods Fifty eight patients who were operated in our hospital during April to Aug.2003 were enrolled.The samples taken from cancer,paracancer or normal tissues of these patients and cancer cell lines(MGC-803,SGC-7901,AGS)were detected for HME protein and HME mRNA expressions by Western blot and immunohistochemistry,RT-PCR and fluorescence quantitative PCR,respectively. Results Both HME mRNA and HME protein expressions were found in all three gastric cancer cell lines.The expressions of HME mRNA and HME protein in gastric cancer tissues was increased signifi- cantly compared with that in normal tissues(P0.05).Conclusions The increased HME expression in gastric cancer tissures compared with normal tissue indicate that HME may be a potential tumor marker for gastric cancer.
