Difference of tumor cell proliferative capability and the interrelated genes'expression in the center and edge tissues of colon cancer
- VernacularTitle:结肠癌中心和边缘组织增殖能力的差异及相关基因的表达
- Author:
Jie GUO
;
Zhi-Xiang SHEN
;
He-Skeng LUO
;
- Publication Type:Journal Article
- Keywords:
Colonic neoplasm;
Gene, p16;
Gene;
p14;
Microdissection;
Methylation
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the difference of tumor cell proliferation between the center and the edge tissues of human colon cancer and its relationship with the expression and methylation of p16 and p14 gene.Methods The expression of Ki67,P14 and P16 protein was assessed by immunohistochemistry,methylation status and mRNA expression of p16 and p14 gene in different area of colon cancer tissue were analyzed by methylation-specific PCR.RT-PCR and microdissection technique. Results There was significant difference in expressions of Ki67 between the center(100.0%) and the edge (30.1%) tissue in 42 cases of colon cancer(P
