Local immune response in gastric mucosa by immunization of H.pylori vaccine with chitosa as adjuvant
- VernacularTitle:以壳聚糖为佐剂的幽门螺杆菌疫苗诱导的黏膜局部免疫反应
- Author:
Yong XIE
;
Nong-Hua LU
;
Yan-Feng GONG
;
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Chitosan;
Vaccine;
Adjuvant
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the adjuvant effects of chitosan on Helicobacter pylori (H.pylori) vaccine.Methods A total of 20 adult mice were randomly divided into four groups.Mice in groups Ⅰ,Ⅱ,Ⅲ, and Ⅳ were orally immunized with PBS,H.pylori antigen alone,H.pylori antigen plus chitosan solution or H. pylori antigen plus chitosan particles,respectively.An ELISA was used to detect anti-H.pylori IgA in saliva and gastric mucosa ,interlukin(IL)-2,IL-4,IL-10 levels in gastric mucosa.Immunohistochemical method was used to detect secretory IgA in gastric mucosa.Results ①The levels of special anti-H.pylori IgA in saliva and gastric mucosa in the groups with chitosan as adjuvant were significantly higher than those in the group without adjuvants and control groups(P
