Integration and expression of HLA-B2704 gene in transgenic mice
- VernacularTitle:人类白细胞抗原B2704基因在转基因小鼠中的整合与表达
- Author:
Zhan-Kun CHEN
;
Hou-Shan LV
;
Dong WANG
;
Dong-Fang JIANG
;
Hai-Ming DING
;
- Publication Type:Journal Article
- Keywords:
Mice, transgenic;
?_2 microglobulin;
Gene expression
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the integration and expression of HLA-B2704 gene in HLA-B2704 transgenic mice. Methods HLA-B2704 gene was introduced into fertilized eggs of C57BL/6?Kunming and Kunming?Kunming by microinjection. The founder mice were screened by PCR for integration of HLA-B2704 transgene and were then further confirmed by southern blot. RT-PCR and flow cytometry were carried out to detect the expression of HLA-B2704 gene at mRNA and protein level. Results Ten F0 hybrid mice carried HLA-B2704 gene in 101 F0 hybrid mice, 3 of 64 (4.7%) hybrid mice from Kunming?Kunming and 7 of 37 (18.9%) hybrid mice from C57BL/6?Kunming background carried HLA-B2704 gene. Statistical analysis showed that there was significant difference in the integration rate between the two groups (P
