EFFECT OF ULTRASONICALLY ACTIVATED HEMATOPORPHYRIN ON EHRLICH ASCITES TUMOR CELLS IN VITRO

VernacularTitle:超声激活血卟啉对腹水型艾氏腹水瘤超微结构的影响
Author: Er-Lin WU ; Yao-Hui REN ; Hao QI ; Wangpan ; Wei TANG ; Quan-Hong LIU ;
Publication Type:Journal Article
Keywords: Apoptosis; Ultrasound; Hematoporphyrin derivative; Transmissim electronmicroscope; Ehrlich ascites tumor; IRC mice
From: Acta Anatomica Sinica 1955;0(03):-
CountryChina
Language:Chinese
Abstract: Objective The aim of this study was to investigate the effect of ultrasonically activated hematoporphyrin on ultrastructure of ehrlich ascites tumor(EAT) cells and to evaluate the potential mechanism of action inducing this cytotoxicity. Methods EAT cells in vitro were exposed to ultrasound at 2^0?MHz and 1^5?W/cm+2 for 3?min in the presence or absence of hematoporphyrin.The changes of ultrastructure of sample preparation for different time were observed by transmission electron microscope. Results The degree of destruction of treated EAT cells was enhanced with the increasing of time for the sample preparation.The sites destroyed mainly involved cell membrane,mitochondrion,endoplasmic reticulum and cell nuclei.Furthermore,morphoiogical characters of ultrasound-activated hematoporphyrin induced apoptosis were observed on EAT cells.Conclusion The killing of tumor cells was ascribed mainly to the damage of ultrastructure induced by ultrasound in combined with hematoporphyrin,apoptosis was also induced during ultrasound and hematoporphyrin killing process.;