Quantitative analysis of total chrysophanol in the processed seeds of Cassia obtusifolia by HPLC
- VernacularTitle:HPLC测定炒决明子中总大黄酚的含量
- Author:
Fang LIU
;
Jianwei CHEN
;
- Publication Type:Journal Article
- Keywords:
Cassia obtusifolia;
chrysophanol;
quantitative analysis;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze the total chrysophanol contents in the processed seeds of Cassia Tora and the contents of chrysophanol in the crude and processed seeds from different districts are compared. METHODS: HPLC with a Kromasil C 18 column was used and MeOH 0.1%H 3PO 4(85∶15) used as the mobile phase. The flow rate was 1.0 mL?min -1 and detection wavelength was 254 nm. RESULTS: The contents of total chrysophanol was 0.196 4% and the average recovery was 96.95%. The content of free chrysophanol was increased after processing, total and combined chrysophanol were decreased. CONCLUSION: The method is reliable of controlling the quality of Cassia obtusifolia and can be used for further studies in the processing of Cassia obtusifolia.
