Determination of three kinds of flavonoid aglycones in the leaves of Acer truncatum bunge by RP-HPLC
- VernacularTitle:不同季节元宝枫叶中的三种黄酮苷元的含量测定
- Author:
Wenhua ZHAO
;
Xiaohong SONG
;
Jun LI
;
Yingxia ZHANG
;
- Publication Type:Journal Article
- Keywords:
Acer truncatum bunge;
flavonoid aglycones;
quercetin;
kaempferol;
isorhamnetin
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To use qualitative and quantitative RP HPLC for the sepatation and determination of flavonoids found in the leaves of Acer truncatum bunge in spring, summer and autumn. METHODS: The determination of three kinds of flavonoid aglycones was carried out with Diamonsil TM C 18 column (4.6 mm?250 mm,5 ?m), using methanol water(1% acetic acid) as mobile phase at a flow rate of 1.0 mL?min -1 and detected at the wavelength of 360 nm. RESULTS: The average recovery of quercetin was 100.4%, RSD =1.61%( n =5). The average recovery of kaempferol was 102.5%, RSD =1.77%( n=5 ). The average recovery of isorhamnetin was 102.3% , RSD =1.63%( n=5 ) CONCLUSION: Isorhamnetin and kaempferol in the leaves are reported here for the first time. The methods were simple, rapid and reliable and could be used to control the quality of the herbal medicines prepared from the leaves of Acer Truncatum Bunge.
