Determination of chlorogenic acid and hesperidin in Qingrehuoxue Mixture
- VernacularTitle:HPLC测定清热活血协定方中绿原酸和橙皮苷的含量
- Author:
Jian-Zhong ZHANG
;
Zhang-Zhang CHEN
;
Qian-Zhou LU
;
Xin GAO
;
- Publication Type:Journal Article
- Keywords:
chlorogenic acid;
hesperidin;
Qingrehuoxue Mixture;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine chlorogenic acid and hesperidin in Qingrehuoxue Mixture (Herba Artemisiae Scopariae, Pericarpium Citri Reticulatae, etc.) at the same time by HPLC. METHODS: A gradient method was set up with eluent of acetonitrile-water (0.4% phosphric acid). The detection wavelength of hesperidin was set at 283 nm, the detection wavelength of chlorogenic acid at 325 nm. RESULTS: The average recovery of two components were all above 95%, RSD was no more than 1.6%. The calibration curve was linear. CONCLUSION:The method is accurate and reliable with good reproducibility.
