Combination of antisense human telomerase RNA and antisense human telomerase catalytic subunit inhibits cervical cancer Hela cell growth
- VernacularTitle:端粒酶RNA反义核酸联合其催化亚单位反义核酸对子宫颈癌Hela细胞生长的抑制作用
- Author:
Lixiu LEI
;
Zhongdong CHEN
;
- Publication Type:Journal Article
- Keywords:
Cervix neoplasms;
Telomerase;
RNA, antisense;
Apoptosis;
Hela cells
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of combination of antisense human telomerase RNA (anti hTR) and antisense human telomerase catalytic subunit (anti hTERT) on proliferation of cervical cancer Hela cell line cultured in vitro Methods Cervical cancer Hela cells were transfected by anti hTR, anti hTERT, anti hTR + anti hTERT, sense hTR, sense hTERT with Oligofectamin TM reagent The proliferation activity of cervical cancer Hela cells was determined using methyl thiazolyl tetrazolium (MTT) The activity of telomerase was tested by polymerase chain reaction telomeric repeat amplification protocol enzyme linked immunosorbent assay (PCR TRAP ELISA) Cell morphologies were observed under fluorescence microscope with acridine orange staining Apoptosis and cell cycle were examined by flow cytometer method (FCM) Results The inhibitory rates on proliferation and apoptotic rates of Hela cells transfected by antisense oligonucleotides were significantly higher compared with those of control, sense oligonucleotides and Oligofectamin TM (all P
