Proteome analysis of indomethacin treated human colon cancer cells
- VernacularTitle:吲哚美辛抗人结肠癌细胞系HCT116的功能蛋白质组研究
- Author:
Yanli CHENG
;
Guiying ZHANG
;
Zhiqiang XIAO
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Indomethacin;
Two-dimensional gel electrophoresis;
Matrix-assisted laser desorption/ionization time of flight mass spectrometry;
Proteome;
Colon cancer
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of indomethacin(IN) on protein maps of human colon cancer cell line (HCT116). Methods Proteome profiles of HCT116 in IN treated and control group were separated by immobilized pH gradient based two dimensional gel electrophoresis (2 DE). Differential expression of protein and proteome analysis were identified and analyzed by silver scan using Image Scanner and ImageMaster software. Peptide mass fingerprint (PMF) was based on matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) and database searching. Results Clear background, well resolution and reproducible 2 DE patterns of HCT116 cells were acquired in IN treated and control group. The protein spots and match rate in control group and IN treated group were 1299?55 (94.2%) and 1208?47 (91.0%) . There were totally 45 differential protein spots, of which the expression of protein in IN treated group was down regulated in 34 spots and up regulated in 9 spots, while 2 spots of protein was expressed only in control group. Twelve differential proteins were identified by PMF, and HCT116 cells apoptosis was through BfL 1 signaling pathway. Conclusion IN can induce HCT116 cells apoptosis though BfL 1 signaling pathway.
