Simultaneous ion pair RP-HPLC determination of oxymatrine and matrine in Compound Kushen Injection
- VernacularTitle:离子对RP-HPLC同时测定复方苦参注射液中氧化苦参碱和苦参碱含量
- Author:
Lei ZHANG
;
Xiaohui CHEN
;
Xi WANG
;
Kaishun BI
;
- Publication Type:Journal Article
- Keywords:
Compound Kushen Injection;
oxymatrine;
matrine;
ion-pair RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: A RP HPLC was developed to determine the contents of oxymatrine and matrine simultaneously in Compound Kushen Injection(Radix Sophorae Flavescentis, Rhizoma Heterosmilacis Japonicae). METHODS: The analysis was carried out on a column of Hypersil ODS 2(4.6mm?200mm,5?m) with a mobile phase of methanol acetonitrile water phosphate acid(10∶30∶65∶0.05,33mmol?L -1 ), flow rate being 1.0mL?min -1 , detection wavelength being at 210nm and the column temperature at room temperature. RESULTS: The linear range was in the range of 17.44～174.4?g?mL -1 ( r =0.9996, n =6) for oxymatrine and in the range of 1.96～ 39.20 ?g?mL -1 ( r=0.9997,n =6) for matrine, respectively. The average recoveries of oxymatrine and matrine were 100.4%( RSD =2.1%, n =9) and 99.7%( RSD =1.4%, n =9), respectively. CONCLUSION: The method is found to be simple and accurate for simultaneous analysis of oxymatrine and matrine in Compound Kushen Injection, and a reliable way to quality evaluation.
