Determination of 4′-O-?-D-glycosyl-5-O-methylvisamminol in Jingfuzhiyang Granules by RP HPLC
- VernacularTitle:HPLC测定荆肤止痒颗粒中5-O-甲基维斯阿米醇苷的含量
- Author:
Decheng ZENG
;
- Publication Type:Journal Article
- Keywords:
Jingfuzhiyang granules;
Herba Schizonepetae;
4′-O-?-D-glycosyl-5-O-methylvisamminol;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a RP HPLC method for the determination of 4′ O ? D glycosyl 5 O methylvisamminol in Jingfuzhiyang Granules (Herba Schizonepetae, Fructus kochiae, Radix Saposhnikoviae, Fructus Crataegi, etc.). METHODS: The sample was separated on Turnner Kromasil C 18 column (4.6mm?200mm,5?m) with mobile phase of methanol 0.05mol?L -1 potassium dihydrogen phosphate acetic acid isopropyl alcohol (85∶170∶4∶4,v/v). The detection wavelength was set at 254nm and the flow rate was 1.0mL?min -1 . The column temperature was 30℃. RESULTS: The calibration curve was linear in the range of 0.0606～0.606?g (r=0.9994). The average recovery was 92.2%. CONCLUSION: This method proves to be relatively simple and accurate, and available for the quality control of Jingfuzhiyang Granules
