The effects of indomethacin on inducing apoptosis and expression of cyclooxygenase-2 and apoptosis regulating genes in human hepatocellular carcinoma cells
- VernacularTitle:吲哚美辛诱导肝癌细胞凋亡及与环氧合酶-2和凋亡调控基因表达关系
- Author:
Yong XIE
;
Nonghua LU
;
Xiaojiang ZHOU
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Indomethacin;
Cyclooxygenase-2;
Hepatocallular carcinoma cell;
Apoptosis
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of non steroidal anti inflammatory drug(NSAID) on apoptosis of hepatocellular carcinoma (HCC)cell lines(SMMC7721) and explore its anti tumor mechanism. Methods HCC line SMMC7721 was treated with different concentration of indomethacin(IN). Apoptosis was identified by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling(TUNEL)method. The expression of cyclooxygenase (COX) 2,p53,p27 kipl , C myc protein and Fas antigen was identified by immunocytochemical SP staining method. Results ①The apoptosis index was (2.4?1.4)%,(4.2? 1.5)% ,(8.0?3.2)%,(14.3?5.1)%,(14.9?4.3)% and (19.0?6.1)%( P0.05 ). Conclusion IN could induce apoptosis of HCC cell line in vitro by down regulating the expression of COX 2 and up regulating the expression of p27 kipl , p53 and c myc.
