Optimization technique in separation of the total flavonoid from Radix Puerariae with macroporous resin
- VernacularTitle:大孔树脂分离葛根总黄酮工艺优化
- Author:
Rongping YANG
;
Binhao WANG
;
Aiquan FANG
;
Qingyao SHOU
;
- Publication Type:Journal Article
- Keywords:
macroporous resin;
total flavonoid of Radix Puerariae;
optimization of technics
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the optimal separation of the total flavonoid from Radix Puerariae by selecting appropriate macroporous resins and to study systematically the factors which affect separation. METHODS: Static and dynamic adsoption desorption methods were adopted, and evaluated for separating efficiency by measuring the concentration of total flavonoid in Radix Pueraria with UV spectrophotometer. RESULTS: The SP70 macroporous resin had the best separating efficiency when the flavonoid content in the liquid was 0.5g per 1mL equivalemt to raw material. pH5～6. the volume of drug 60BV (resin bed volume) with the adsorption power 2BV/h. and the volume of 70%(mL?mL -1 ) ethanol as eluant 4BV with desorption power 2BV/h. In result. total flavonoid's purity could reach 80%. CONCLUSION: This method is simple and feasible with good effect of separation, which can meet industrial requirements.
