Determination of astragaloside Ⅳ in Qixiu Granules by HPLC-ELSD
- VernacularTitle:HPLC-ELSD测定芪休颗粒中黄芪甲苷的含量
- Author:
Jinkui TIAN
;
Limin WU
;
Jianjian LIU
;
Aiwu WANG
;
Hui GEN
;
Hongmei LIU
;
- Publication Type:Journal Article
- Keywords:
Qixiu Granule;
HPLC ELSD;
astragaloside Ⅳ
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine astragaloside Ⅳ in Qixiu Granule (Radix Astragali seu Hedysari, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, etc.) by HPLC ELSD. METHODS: HPLC ELSD was used in the quantitative analysis by using Alltima C 18 chromatography column and acetonitril water (34∶66) as a mobile phase. The flow rate of mobile phase was 1mL?min -1 . The tube temperature of the detector was 100?C. The flow rate of pure air was 2.7L?min -1 . RESULTS: The regressione quation was Y=-4.40?105+2.07?105X, r=0.9992 (3.73～13.59?g). The average recovery of astragaloside Ⅳ was 100.73%, RSD was 3.97%. CONCLUSION: The method is simple, reliable, accurate and can be regard as the quantity control method of Qixiu Granule.
