Effect of curcumin on lipid peroxidation of oxidative stress in rat stellate cell of liver
- VernacularTitle:姜黄素抗大鼠肝星状细胞氧应激脂质过氧化作用的研究
- Author:
Yonggang LIU
;
Yongzhong LIU
;
Xiaodong WANG
;
- Publication Type:Journal Article
- Keywords:
curcumin;
hepatic stellate cells;
lipid peroxidation
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of antioxidant curcumin on the lipid peroxidation of oxidative stress in rat hepatic stellate cell in vitro. METHODS: HSC T6 were incubated with 0.1mM ferric nitrilotriacetate complex (FeNTA), MTT colorimetry was used for assaying proliferation of HSC, Cytotoxicity was measured by LDH colorimetry, Collagen type Ⅰ accummulation in the culture media was measured by ELISA, Intracellular malonildialdehyde (MDA)、SOD、GSH and GSH PX levels in the culture media were measured by their reagent boxes. RESULTS: HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decrease SOD and GSH PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type Ⅰ levels in the culture medium. All these effects were reversed by the antioxidant curcumin. CONCLUSION: The mechanism of curcumin on fibrosis may be related to inhibit lipid peroxidation
