Determination of Geniposide in Yigan Liangcha by RP-HPLC
- VernacularTitle:RP-HPLC法测定益肝凉茶中栀子苷的含量
- Author:
Dong'Ai YANG
;
Xiangdong DAI
;
- Publication Type:Journal Article
- Keywords:
Yigan Liangcha;
Geniposide;
RP-HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To establish a RP-HPLC method for the determination geniposide in Yigan Liangcha.Methods Luna C18 (4.6 mm?250 mm,5 ?m)was applied,the mobile phases consisted of acetonitrile-water(15∶85),the flow rate was 1.0 mL?min-1 and the detection wavelength at 238 nm.Results The average recovery of geniposide was 98.1 %and RSD were 0.42 %(n=5).There is a good linear relationship within the range of 10.12～50.60 ?g of geniposide.Conclusion The method is convenient,sensitive,accurate and reproducible and can be used the quality control of the Yigan Liangcha.
