Role of cytokine RhoA in the formation of hepatic fibrosis in rats
- VernacularTitle:细胞因子RhoA在实验性大鼠肝纤维化形成过程中的作用机制
- Author:
Bo WANG
;
Tiancai WANG
;
Kuohuan LIANG
;
- Publication Type:Journal Article
- Keywords:
RhoA;
Stress fiber;
Vinculin;
Hepatic fibrosis
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression changes of cytokine RhoA,stress fiber and Vinculin in the formation of hepatic fibrosis in rats, and investigate the role of RhoA in hepatic fibrosis. Methods Forty male SD rats were divided into two groups:model group and control group. The rats in the model group were given 60% CCl 4 by subcutaneous injection(1 ml/kg). The liver tissue was taken out for research at 1st、4th and 8th week respectively, RhoA and Vinculin mRNAs were detected by RT PCR, Vinculin and F actin proteins were detected by immunohistochemistry and immunofluorescence respectively, RhoA and Vinculin proteins were detected by Western blotting. Results Compared with control group, the expressions of mRNA of RhoA and Vinculin, the proteins of Vinculin, F actin and RhoA were all significantly increased( P
