Possible signal pathway of the apoptosis in gastric cancer cell lines induced by arsenic trioxide
- VernacularTitle:氧化砷诱导胃癌细胞凋亡的信号传导途径研究
- Author:
Qin CAO
;
Shuiping TU
;
Jihong TAN
;
Tianle MA
;
Minmin QIAO
;
Yuxin WU
;
Shihu JIANG
;
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Gastric cancer, Apoptosis;
Signal pathway
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible signal pathway of the apoptosis in gastric cancer cell lines(SGC 7901 and MKN 45)induced by arsenic trioxide. Methods TUNEL method was used to observe the influence of calcium antagonist, inhibitors of protein kinase C (PKC) and protein tyrosine kinase(PTK) on the apoptosis of gastric cancer cells induced by arsenic trioxide. Levels of cAMP, PKC and PTK were detected before and after the treatment with arsenic trioxide. Results Both PKC and PTK inhibitors could induce apoptosis of gastric cancer cell lines, also both of them had a cooperative action with arsenic trioxide in inducing apoptosis of gastric cancer cells, while calcium antagonist had no any effect on the apoptosis of gastric cancer cell lines. PKC and PTK levels decreased but cAMP level increased during the apoptosis of gastric cancer cells induced by arsenic trioxide ( P
