Determination of Tanshinone Ⅱ_A and Schizandrol A in Anshenbuxin Pill by HPLC
- VernacularTitle:HPLC测定安神补心丸中五味子醇甲和丹参酮Ⅱ_A含量
- Author:
Ying LI
;
Zhongliang CHEN
;
- Publication Type:Journal Article
- Keywords:
Anshenbuxin Pill;
tanshinone Ⅱ A;
schizandrol A;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill(Radix Salviae Miltiorrhizae, Fructus Schisandrae Chinensis, Rhizoma Acori Tatarinowii,etc.). METHODS: HPLC was used to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill. The separation was performed on C 18 column with methanol water (80∶20) for Tanshinone Ⅱ A and methanol water(50∶50) for Schizandrol A, respectively. The detection wavelength was at 270nm for Tanshinone Ⅱ A and 250nm for Schizandrol A, respectively. RESULTS: The average recovery of Tanshinone Ⅱ A was 98.54%( RSD =1.75%, n =6) and the average recovery of Schizandrol A was 99.05%( RSD =1.77%, n =6). CONCLUSION: This method is simple, accurate with good reproducibility and can be used to control the quality of Anshenbuxin Pill.
