Determination of hydrochlorothiazide and rutin in Zhenjujiangya Tablets by HPLC
- VernacularTitle:HPLC测定珍菊降压片中氢氯噻嗪和芦丁的含量
- Author:
Huali YAN
;
Shuguang JI
;
Anjun HUANG
;
- Publication Type:Journal Article
- Keywords:
HPLC;
content;
hydrochlorothiazide;
rutin
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method for determination of hydrochlorothiazide and rutin in Zhenjujiangya Tablets(Flos Chrysanthemi Indici, hydrochlorothiazide, rutin, etc.) by HPLC. METHODS: HPLC was used with Spherisorb C 18 (4.6mm?250mm 5?m) column 0.05mol?L -1 citric acid acetonitrile(80∶20) (adjusted the mixture with triethylaminum to pH 4.1?0.1) was used as a mobile phase. The flow rate was 1.0mL?min -1 . The detection wavelength was at 270nm. RESULTS: The linear response range lay in 10～50?g?mL -1 of hydrochlorothiazide( r =0.9994 n =5) and 40～200?g?mL -1 of rutin( r =0.999 n =5), respectively. The average recoveries of hydrochlorothiazide and rutin were 99.8% and 99.5%, respectively. CONCLUSION: This method is rapid, accurate and suitable for the determination of the two constitutents in the compound tablets.
