Determination of emodin and chrysophanol in Fuyanling Effervescent Tablets by HPLC
- VernacularTitle:HPLC测定妇炎灵泡腾片中大黄素、大黄酚的含量
- Author:
Ying YU
;
Ling DONG
;
Qiuxia DU
;
Honghui JIA
;
Fugui LI
;
- Publication Type:Journal Article
- Keywords:
Fuyanling Effervescent Tablets;
emodin;
chrysophanol;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method to determine the content of emodin and chrysophanol in Fuyanling Effervescent Tablets(Radix et Rhizoma Rhei, Flos Lonicerae, Radix Sophorae Tonkinensis, etc.). METHODS: HPLC was used to determine the content of emodin and chrysophanol in Fuyanling Effervescent Tablets. The separation was performed on YWG ODS column with methanol (0.1%) phosphoric acid(85∶15) mixture as a mobile phase and the wavelength of UV detector was at 254nm. RESULTS: The resolution and the linearity of this method was good. in the range of emodin was 50.2～401.6ng( r =0.9999); chrysophanol was 49.9～ 399.2 ng( r =0.9994); and with the average recovery of emodin: 99.4%( RSD =2.2%); chrysophanol:100%( RSD =1.1%). CONCLUSION: The method is simple, rapid and satisfactory and suitable for quality control of Fuyanling Effervescent Tablets.
