Effects of exogenous brain-derived neurotrophic factors on concentrations of cerebral endogenous opioid peptides in neonatal rats subject to with hypoxic-ischemic brain injury
- VernacularTitle:脑源性神经营养因子对新生大鼠缺氧缺血性脑损伤后内源性阿片肽的影响
- Author:
Xinru HONG
;
Xiaoxin LU
;
Xinmin CHEN
;
- Publication Type:Journal Article
- Keywords:
Neurotrophic factor;
Opioid peptides;
Brain hypoxia-ischemia;
Rat, newborn
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of brain derived neurotrophic factors (BDNF) on concentrations of cerebral endogenous opioid peptides(EOP)in neonatal rats subject to with hypoxic ischemic brain injury (HIBI). Methods Seven day old Sprague Dawley rats were randomly divided into a HIBI+BDNF group (group A),a HIBI group (group B) and a sham operation group (group C). Models of HIBI were established by use of permanent ligation of the left common carotid artery followed by 2.5 hours ofinhalation of 8%O 2+92%N 2, then 0.5 ?g BDNF was injected into the parietal cerebral ventricle in group A immediately. Contents of dynorphin A 1 13 like, ? endorphin like and leu enkaphalin like immunoreactivities (ir DynA 1 13 , ir ? EP and ir LEK) in cortex and hippocampus were measured at 0, 60, 120 min after administration of BDNF. Results The concentrations of ir DynA 1 13 and ir ? EP in the cortex and hippocampus in group B were increased significantly than those in group C at most time points( P
