The biological properties of decellularized human tissue-engineering aortic valves conduits
- VernacularTitle:去细胞组织工程同种心脏瓣膜的生物学特征
- Author:
Bin CUI
;
Yinglong LIU
;
Ning XIE
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Heart valve prosthesis Bioprosthesis Biology Tissue engineering
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the biological properties of a decellular human tissue engineering homograft aortic valves conduits. Methods: (1) Human homograft aortic valves conduits were treated with the pH 8 0 hypotonic tris buffer for 12h. (2) Then with pH 8 0 isotonic buffer added with the 1% DOA (deoxycholic acid) for 24h. (3) At last, a pH 7 6 isotonic buffer with the DNAase 200?g/ml, NAase 20 ?g/ml was applied. The histology, water content, thickness, denaturation temperature, DNA content, collagen contents were examined. Results: The valves, the wall and the muscle layer of the human homograft aortic valves conduits were decellulared completely compared with the standard crypreserved human homograft aortic valves. Water content in the decellular aortic wall was increased significantly (75 44?1 84 vs. 82 05?0 71,P
