Determination of puerarin and paeoniflorin in Jingfukang Granules
- VernacularTitle:HPLC同时测定颈复康颗粒中葛根素、芍药苷的含量
- Author:
Kewei ZHANG
;
Wei LI
;
Yumei CHI
;
Xiaobing CUI
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Jingfukang Granules;
dual wavelength;
puerarin;
paeoniflorin;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for determination of puerarin and paeoniflorin in Jingfukang Granules(Radix Puerariae, Radix Paeoniae Alba, etc.). METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol water. Puerarin and paeoniflorin were determined by HPLC(dual wavelength, ? 1=250nm,? 2=230nm). RESULTS: The linear range of puerarin was within 99.6ng～996.0ng, r =0.9998, sample recovery was 100.69%, RSD =1.04%( n =9), respectively. The linear range of paeoniflorin was within 119.6ng～ 1315.6 ng, r =0.9999, sample recovery was 100.30%, RSD =1.24%( n =9), respectively. CONCLUSION: This method is simple, reliable and has good repeatability. It can be used for quality control of Jingfukang Granules in production.
