Effect of Peach seed protein A on gene expression in mouse with S_(180)
- VernacularTitle:用基因芯片研究桃仁蛋白A对小鼠纤维肉瘤基因表达的影响
- Author:
Ying LIU
;
Yajie LI
;
Liying ZHU
;
- Publication Type:Journal Article
- Keywords:
protein A, Semen Persicae;
antitumor effect;
DNA microarray
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of peach seed (semen Persicae) on gene expression of mouse with sarcoma 180 by means of DNA microarray. METHODS: DNA microarrays were made by spotting PCR products of 4096 mice cDNA noto a specially treated glass slides. The probes were prepared by labeling experimental and control group tumor tissue mRNA with Cy3 dUTP and Cy5 dUTP separately through reverse transcription. The arrays were then hybridized against the cDNA probe mixture and the fluorescent signals were scanned. The data obtained were analyzed from two repeated experiments. RESULTS: 64 genes were obtained by computer processing. CONCLUSION: These genes identified through this approach are considered as potential candidates for PSPA anti tumor effect.
