Determination of the fingerprint chromatogram of Hongjingtian Injection by HPLC
- VernacularTitle:红景天注射剂指纹图谱的HPLC研究
- Author:
Guiyan ZHANG
;
Zaohua ZHANG
;
Jinong CHU
;
- Publication Type:Journal Article
- Keywords:
Hongjingtian Injection;
Rhodiola kirilowii (Regel) Maxim;
fingerprint chromatogram;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the fingerprint chromatogram of Hongjingtian Injection ( Rhodiola kirilowii (Regel.) Maxim. METHODS: HPLC with YWG C 18 column was used, the (a)MeOH ACN(1∶1)、(b) 0.07% H 3PO 4 H 2O gradient elution as a mobile phase and detection wavelength at 278nm . RESULTS: 17 peaks were indicated on the HPLC fingerprint of Hongjingtian Injection. The relative retention time and relative peak area were obtained with itself peak. CONCLUSION: The method is simple and accurate with good reproducibility and can be used as a quality control method of Hongjingtian Injection.