Effects and mechanism of celecoxib on rat colitis induced by trinitrobenzene sulfonic acid
- VernacularTitle:选择性环氧合酶-2抑制剂塞来昔布在实验性结肠炎中的作用机制
- Author:
Ling ZHANG
;
Yumin LU
;
Xiuyun DONG
;
- Publication Type:Journal Article
- Keywords:
Experimental colitis;
Selective cyclooxygenase 2 inhibitor;
Prostaglandin;
Celecoxib
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective An investigation was conducted to assess the effects and mechanism of celecoxib [a selective cyclooxygenase(COX) 2 inhibitor] on a rat colitis model induced by trinitrobenzene sulfonic acid (TNBS). Methods The rats were divided into four groups. Group 1 and group 2 were experimental groups. Group 3 and group 4 were control groups. Colitis was induced by intracolonic administration of TNBS (25 mg/ml) in a vehicle of 50% ethanol (0.25 ml) in rats of experiment groups. Three hours before induction of colitis ,the rats were beginning and continuing to treat orally with celecoxib (1.25 mg/kg, group 1) and distilled water (1 ml/0.3 kg, group 2) twice per day for 7 days , respectively. The rats in group 4 were treated orally with celecoxib (1.25 mg/kg) twice per day for 7 days. Group 3 served as healthy control. All rats that survived until the end of the experiment (7 d) were killed and the severity of damage were assessed. The prostaglandin E2 (PGE2) concentrations of colonic mucosa were tested by radioimmunoassay. Results The colonic damage scores were 11.15?3.3 in group 1 and 8.50?2.82 in group 2. Both were significantly higher than that of group 3 (0.62?0.09)( P
