Effecting of inhibiting ubiquitin-proteasome pathway on proliferation and apoptosis of gastric carcinoma cells
- VernacularTitle:阻断泛素-蛋白酶体通路对胃癌细胞增殖和凋亡的影响
- Author:
Qingming WU
;
Jieping YU
;
Weiguo ZHANG
;
Xianjun LIU
;
Qiang TONG
;
- Publication Type:Journal Article
- Keywords:
Ubiquitin proteasome;
Gastric carcinoma;
Cell cycle;
Apoptosis;
Telomerase
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhibiting ubiquitin proteasome pathway(UPP) on proliferation of gastric carcinoma cells and the possible mechanism was discussed. Methods The gastric carcinoma cell strain SGC 7901 was treated with MG 132 to inhibit its UPP specially. The effect of growth suppression on cells was evaluated with MTT assay. Cell cycle and apoptosis were detected by flow cytometry(FCM). DNA fragment analysis was used for confirming the presence of apoptosis. The activity of telomerase was examined by TRAP PCR ELISA. Expression of p27kip1 was detected by immunocytochemical technique. Results MG 132 had great inhibitory effect on the growth of SGC 7901 cells. The FCM analysis showed that the ratio of G0/G1 phase of control group was (46.3?4.1)%, the ratio of G0/G1 phase of SGC 7901 cells treated with MG 132 increased to (72.1?5.0)% ( P
