Study on extraction of coumarins in Angelica Dahurica by supercritical CO_2
- VernacularTitle:超临界CO_2萃取白芷中香豆素类成分的工艺优化研究
- Author:
Hongmei LIU
;
Mingxian ZHANG
;
Yangping LIU
;
Yongping HAN
;
Fengyun TAO
;
- Publication Type:Journal Article
- Keywords:
supercritical extraction process;
Angelica Dahurica;
coumarins;
orthogonal design;
stepwise regression analysis
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To optimize the extraction process for coumarins in Angelica Dahurica by supercritical CO 2 as guided by the contents of oxyimperatorin, imperatorin and the total coumarins in the extract. METHODS: HPLC was applied to determine the contents of oxyimperatorin and imperatorin. orthogonal design was applied to optimize SFE process. Range and variance analysis as well as stepwise nonlinear regression analysis were applied to deal with experimental results. RESULTS: The optimum process was established as following: 21MPa as extracting pressure, 50℃ as extracting temperature, 3.5h as extracting time, 20 mesh as pulverized degree and 6.5MPa as separating pressure. The relative error between experimental data and calculated value was less than 5%. CONCLUSION: Changes in extracting pressure, temperature, time, pulverized degree would affect the extracting results remarkably. Some factors had interactions on the extraction of coumarins in Angelica Dahurica . Regression equation was reasonable and could forecast results precisely.
