Dtermination of emodin and chrysophanol in Zhenhuang Powder by RP-HPLC
- VernacularTitle:RP-HPLC测定珍黄散中大黄素和大黄酚的含量
- Author:
Renliu XU
;
Huiying LIU
;
- Publication Type:Journal Article
- Keywords:
Zhenhuang Powder;
emodin;
chrysophanol;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: A methond was established for the determination of emodin and chrysophanol in Zhenhuang Powder (Margarita, Radix et Rhizoma Rhei, artificial Calculus Bovis, etc.). METHODS: RP HPLC was used for samples separated on a shim pack VP ODS column with the mobile phase of methanol-0.1% H 3PO 4(85∶15) and the detection wavelength was set at 430nm. RESULTS: The calibration curves were linear in the range of 0.01181～4.724?g( r =0.9994) for emodin and in the range of 0.01300～5.200?g( r =0.9999) for chrysophanol, respectively. The average recoveries were 99.0%( RSD =1.23%, n =5) and 100.5% ( RSD =1.06%, n =5), respectively. CONCLUSION: The method is simple, realizable and reproducible. It can be used effectively for the quality control of Zhenhuang Powder.
