Preparation and purification of theanine reference substance by preparative HPLC
- VernacularTitle:制备高效液相色谱分离纯化茶氨酸对照品
- Author:
Weitao XIAO
;
Xiaolan ZHU
;
Bo CHEN
;
Shouzhuo YAO
;
- Publication Type:Journal Article
- Keywords:
theanine;
reference substance;
preparative HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish an isolation method of theanine reference substance by preparative HPLC. Methods After raw material extracted by water, the water phase was extracted by chloroform saturated with water, then concentrated on a thermostat. After centrifuged, supernatant was isolated and purified by preparative HPLC. Fraction was frozen and dried by Flexi Drier. Crude product was rinsed by methanol. The purity of product was determined by analytical HPLC. Results The purity of product is higher than 98% and theanine yield from raw material by this method exceeds 60%. Conclusion The developed method is simple, rapid, and at low production cost. The product owns the quality of reference substance.
