HPLC determination of ethyl p-hydroxybenzoate in Dragon's Blood
- VernacularTitle:HPLC测定龙血竭中对羟基苯甲酸乙酯的含量
- Author:
Qingyun ZHANG
;
Langui CUI
;
Yingqing HU
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Dragon's Blood;
ethyl p hydroxybenzoate
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish a HPLC method for determination of ethyl p hydroxybenzoate in Dragon's Blood. Methods Using C 18 reverse phase column and acetonitrile 1% acetic acid (31∶69) as mobile phase, detecting at 257 nm and quantitating with external standard method. Results The standard curves of ethyl p hydroxybenzoate showed good linearity over the range of 0.206-2.884 ng, r=0.999 8. The average recovery was 96.02% and RSD was 1.87%. Conclusion The method is quick and simple, and it is accurate and reliable for the determination of ethyl p hydroxybenzoate in Dragon's Blood.