Impacts of the transfection of antisense DNA methyltransferase Ⅰ gene fragment into hepatocellular cell on its sensitivity to tumor necrosis factor related apoptosis inducing ligand
- VernacularTitle:反义甲基转移酶基因片段增强肿瘤坏死因子相关凋亡诱导对肝癌细胞的作用
- Author:
Xiaoan LI
;
Dianchun FANG
;
Hong ZHANG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Hepatocellular cells;
DNA methyltransferase Ⅰ gene fragment;
Apoptotic rate;
Survival cell rate
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impacts of the transfection of antisense DNA methyltransferase Ⅰ(DNMT1) gene fragment into SMMC 7721 cell on the changes of cell sensitivities to tumor necrosis factor related apoptosis inducing ligand(TRAIL). Methods The eukaryon expression vector pCl neo was transfected into SMMC 7721 cells by liposomes and the transfection was identified by PCR. Survival cell rate was measured by trypan blue exclusion, apoptotic rate was determined by in situ TdT dUTP terminal nick end labeling(TUNEL) method. Results PCR detection indicated that the eukaryon expression vector pCl neo was successfully transfected into SMMC 7721 cells. Survival cell rate of SMMC 7721 cells transfected with antisense DNMT1 gene fragment was remarkably lower than that of transfected with sense DNMT1 gene fragment and empty vector ( P
