Role of mast cell in rectal hypersensitivity induced by acute stress in rats
- VernacularTitle:肥大细胞在应激所致大鼠直肠高敏感性中的作用
- Author:
Yaozong YUAN
;
Yanbing LIU
;
Ranjun TAO
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Irritable bowel syndrome;
Restraint stress;
Visceral hypersensitivity;
Mast cell
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been know that visceral hypersensitivity is one of the important features of irritable bowel syndrome(IBS) and psychological factors may be implicated in the etiology of IBS. The aim of our study was to investigate the influence of an acute psychological stress on the rectal sensitivity in rats and the role of mast cell in this response. Methods A stress model was established by mild restraint lasting 2 hours and the effect of acute stress on visceral sensitivity to rectal distension(RD) was measured by abdominal electromyography. The number of mast cells was counted and mast cell activation was determined by histamine release after in vitro stimulation with substance P(SP) in colonic pieces from stressed and control rats. We also studied the effect of acute stress on somatic nociception and colonic transit. Results Abdominal response to RD for all volumes of distension(0.5, 0.8, 1.2, 1.6 ml)was significantly enhanced by acute restraint stress compared with sham stress( P 0.05 ). Acute stress also stimulated colonic transit and produced significant somatic analgesia. Conclusion Acute stress enhanced rectal sensitivity in response to rectal distension and colonic mast cell activation may be involved in this response.
